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Doctoral position in Biophysics in high resolution X-ray imaging of nematodes.
Research and graduate education at the department of chemistry and molecular biology comprises a wide scientific field from the atomic and molecular levels all the way through cells to intact organisms. Much of the phenomena studied here impact on our natural environment and living systems. We participate in undergraduate programs in chemistry, molecular biology, medicinal chemistry, biology, marine sciences, and pharmacy.
With support from the Knut and Alice Wallenberg foundation, Richard Neutze is working with Julia Morud Lekholm and Johanna Höög towards the goal of understanding how an evolved biological neural network functions to processes information. The neuronal network of study is Caenorhabditis elegans, a roundworm or nematode, which is the simplest known model system in neuroscience. The hermaphrodite animal has 302 neurons. A key technology within the Wallenberg Scholar project is X-ray imaging of the neural connectome (the connections between neurons) of Caenorhabditis elegans. This PhD position therefore aims to use X-ray imaging to recover structural information on the spatial arrangements of, and the connections between, neurons in roundworms. The planned research will be to develop the foundations for very high-resolution X-ray imaging of biological samples by using high-pressure freezing methods to prepare nematode samples, to embed these frozen nematodes in epoxy resin and to harden them, to evaluate the quality of sample preparation process using transmission electron microscopy, to participate in X-ray imaging of these samples at synchrotron radiation facilities, and to lean image processing and computer aided reconstruction methods. A variety of resins will be tested to evaluate the quality of the embedding step. Once robust, high quality sample preparation and analysis protocols have been developed, the goal is to reconstruct the connectome of Caenorhabditis elegans from an entire intact animal using synchrotron radiation. Machine learning methods will be utilized when performing this reconstruction. As such, this PhD position combines both preparative and computational tools and uses synchrotron radiation for structural analysis.
General information about being a doctoral student at the University of Gothenburg can be found on the university's doctoral student pages.
https://www.gu.se/en/doctoral-studies
Duties
The PhD student will work towards developing tools for very high-resolution X-ray imaging of biological samples using synchrotron radiation. This work involves:
- Using high-pressure freezing methods to prepare nematode samples.
- Embedding frozen nematodes in epoxy resin and hardening them.
- Evaluating the quality of sample preparation process using transmission electron microscopy.
- Participating in X-ray imaging of these samples at synchrotron radiation facilities.
- Applying image processing computer packages to analyze these data and generate three-dimensional reconstructions.
- Use machine learning tools to trace connections between neurons in three-dimensional reconstructions.
Depending upon the interests of the student appointed, the work may also include supportive work on protein targets from Caenorhabditis elegans, or simulations of the neuronal connectome of the animal.
Please note, doctoral studies at The Department of Chemistry and Molecular biology require physical presence to conduct the studies. If the admitted applicant needs a residence permit for higher education to pursue studies in Sweden, The Department of Chemistry and Molecular biology has the right to revoke the admission decision if the applicant cannot present a valid residence permit no later than at the start of the studies.
Contact information
Richard Neutze: [email protected]
Julia Morud Lekholm: [email protected]
Johanna Höög: [email protected]
Unions
Union representatives at the University of Gothenburg can be found here:
https://www.gu.se/om-universitetet/jobba-hos-oss/hjalp-for-sokande
Application
You can apply to be admitted to doctoral education via the University of Gothenburg’s recruitment portal.
It is your responsibility to ensure that the application is complete as per this notice, and that the University receives it by the final application deadline.
You must include the following, in PDF format
- Personal letter
- CV
- Diploma and transcripts showing that you meet with the general entry requirement
- Proof of completion of English 6 course or the acquired equivalent knowledge through previous studies
- Two references (name, e-mail, telephone number, relation)
If your degree has not been verified by a Swedish authority you need to provide contact details to the issuing university, registrar or similar, that can verify the degree at our request.
Applications must be received by: 2025-07-10
Information for International Applicants
Choosing a career in a foreign country is a big step. Thus, to give you a general idea of what we and Gothenburg have to offer in terms of benefits and life in general for you and your family/spouse/partner please visit:
https://www.gu.se/en/about-the-university/welcome-services
https://www.movetogothenburg.com/
The University works actively to achieve a working environment with equal conditions, and values the qualities that diversity brings to its operations. Salaries are set individually at the University. In accordance with the National Archives of Sweden’s regulations, the University must archive application documents for two years after the appointment is filled. If you request that your documents are returned, they will be returned to you once the two years have passed. Otherwise, they will be destroyed. In connection to this recruitment, we have already decided which recruitment channels we should use. We therefore decline further contact with vendors, recruitment and staffing companies.
